Pyronin Y
SIGMA/P9172 - for NA electrophoresis
Synonym: Pyronin Y; Pyronin G; Pyronin J
CAS Number: 92-32-0
Empirical Formula (Hill Notation): C17H19ClN2O
Molecular Weight: 302.80
EC Number: 202-147-8
MDL Number: MFCD00011725
Linear Formula: C17H19ClN2O
Product Type: Chemical
| composition | Dye content, ≥45% |
| form | powder |
| grade | Molecular Biology |
| InChI | 1S/C17H19N2O.ClH/c1-18(2) |
| InChI key | INCIMLINXXICKS-UHFFFAOYSA |
| mp | 250-260 °C (lit.) |
| Quality Level | 200  |
| SMILES string | [Cl-].CN(C)c1ccc2C=C3C=C |
| solubility | water: 10 mg/mL, dark red |
| storage temp. | room temp |
| suitability | suitable for nucleic acid staining |
| technique(s) | nucleic acid detection: suitable |
| Application: | Pyronin Y has been used: • in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs) • as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs) • staining of CD34+ cells |
| Biochem/physiol Actions: | Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells. In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections. |
| Packaging: | 1, 5 g in glass bottle |
| Preparation Note: | This product is soluble in water 1 mg/ml, yielding a clear dark red solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 250-260 °C (lit.) |
| Storage Temp. | room temp |
| Colour Index Number | 45005 |
| UNSPSC | 12171500 |