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SIGMAFAST™ OPD

SIGMA/P9187 - tablet

Synonym: OPD Peroxidase Substrate

Product Type: Chemical
Catalog Number PKG Qty. Price Quantity
45-P9187-5SET 5 sets
 $59.40
1/EA		 Add To Favorites
45-P9187-50SET 50 sets
 $294.00
1/EA		 Add To Favorites
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.

 

form tablet
Quality Level 200 
solubility water: 0.5 tablet/10 mL, clear, colorless
storage temp. 2-8°C
Application: SIGMAFAST™ OPD (o-Phenylenediamine dihydrochloride) tablets have been developed for use as a soluble substrate for the detection of peroxidase activity in enzyme immunoassays (EIA). OPD is the EIA substrate of choice as it exhibits high sensitivity. EIA applications utilizing OPD may be read in timed assays or stopped with dilute acid solutions for delayed readings.
SIGMAFAST OPD has been used in saliva- virus-like particles (VLP) binding assay. It has also been used to develop enzyme linked immunosorbent assay (ELISA) plates for the detection of rituximab in rat serum samples.
Disclaimer: This substrate is hygroscopic and light sensitive
Features and Benefits: SIGMAFAST™ OPD Tablets require no additional buffers or steps to prepare an active substrate solution.
Legal Information: SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
Preparation Note: Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing OPD and urea hydrogen peroxide.
Preparation Note: One OPD tablet and one urea hydrogen peroxide/buffer tablet, dissolved in 20 ml of water, provides 20 ml of ready-to-use substrate. When the tablet is dissolved in this manner, the final concentrations will be 0.4 mg/ml OPD, 0.4 mg/ml urea hydrogen peroxide, and 0.05 M phosphate-citrate, pH 5.0. Tablets are individually packaged in foil packets.
Storage Temp. 2-8°C
UNSPSC 12352204

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