Phospholipase A2 from honey bee venom (Apis mellifera)
SIGMA/P9279 - salt-free, lyophilized powder, 600-2400 units/mg protein
Synonym: Lecithinase A; PLA2; Phosphatidylcholine 2-acylhydrolase
CAS Number: 9001-84-7
EC Number: 232-637-7
MDL Number: MFCD00131890
Product Type: Chemical
Phospholipase A2 reacts stereospecifically with most sn-3-phosphoglycerides. The fatty acid ester bonds are hydrolyzed at the C-2 position.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P9279-25MG
| form | salt-free, lyophilized powder |
| mol wt | 14.5 kDa |
| Quality Level | 200  |
| specific activity | 600-2400 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P00630  |
| Analysis Note: | Protein determined by biuret. |
| Application: | Phospholipase A2 from honey bee venom (Apis mellifera) has been used • to determine renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation • in the treatment of photosystem-II (PSII) dimer complex of Synechocystis to study changes in oxygen-evolving activity • as a standard in phospholipase activity assay |
| Biochem/physiol Actions: | Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells. |
| General description: | Phospholipase A2 from honey bee venom (Apis mellifera) is a calcium-dependent lipolytic enzyme. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of soybean |
| Packaging: | 1, 5, 25 mg in glass bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 600-2400 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.1.1.4 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |