Proteinase K–Agarose from Tritirachium album
SIGMA/P9290 - lyophilized powder
Synonym: Proteinase K-Agarose; Proteinase K-Agarose
MDL Number: MFCD00132131
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P9290-10UN
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: P9290-25UN
| biological source | microbial (T.album T. ALBUM) |
| extent of labeling | 30-140 units per g solid |
| form | lyophilized powder |
| Quality Level | 200  |
| storage temp. | −20°C |
| swelling | 1 g swells to ~5 mL |
| technique(s) | DNA extraction: suitable |
| protein extraction: suitable | |
| RNA extraction: suitable |
| Application: | Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs. |
| Biochem/physiol Actions: | The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column. |
| Features and Benefits: | • Recylable Proteinase K on matrix support. • Spin column or batch processing possible. |
| Other Notes: | One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent). |
| Packaging: | 10, 25 units in glass bottle |
| Physical form: | Lyophilized powder stabilized with lactose |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106500 |