Peroxisome Isolation Kit
SIGMA/PEROX1 - isolate peroxisomes from tissues and cells
Synonym: Isolation Kit for Peroxisomes; Kit for Peroxisomes; Peroxisome Kit
Product Type: Chemical
SDS-PAGE Rat liver peroxisomes were purified according to the isolation procedure. Samples of 0.2 μg protein from the different purification stages/fractions were run on 7.5% SDS-PAGE. Immunoblotting was performed using anti-PMP70 at a dilution of 1:2000. Lanes: A: 1,000 × g supernatant. B: The endoplasmic reticulum fraction from the OptiPrep™ gradient. C: The fraction containing mainly mitochondria (with some peroxisomes) from the OptiPrep™ gradient. D: The purified peroxisome fraction from the OptiPrep™ gradient.
| Quality Level | 200  |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| technique(s) | centrifugation: suitable |
| fractionation: suitable |
| Application: | The Perixosome Isolation Kit provides all the necessary reagents and a detailed protocol for the isolation of highly purified peroxisomes from animal tissues and cells, by differential density gradient centrifugation using iodixanol [OptiPrep™]. This kit has been used for preparation of peroxisomes from rat liver, rat kidney and rabbit liver as well as HEK293 and HepG2 cells. |
| Features and Benefits: | • Specially formulated extraction reagents for research scale applications - save time and minimize waste • Produces functional intact organelles - resulting peroxisomes are suitable for functional studies, metabolic assays, protein profiling, and disease state analysis • Compatible with products for structure confirmation - easily confirm intactness with companion test kit, Cytochrome C Reductase Assay Kit (Cat. No. CY0100) |
| General description: | Isolated peroxisomes are used for studying lipid β-oxidation, amino acid metabolism and biosynthesis of ether-linked glycerolipids and bile acids. |
| Legal Information: | OptiPrep is a trademark of Serumwerk Bernburg AG |
| Other Notes: | Upon receiving the kit, the Protease Inhibitor Cocktail (Product Code P 8340) should be stored at –20 °C and the OptiPrep Density Gradient Medium (Product Code O3028) should be stored at room temperature. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 |
| Precautionary statements | P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313 |
| Hazard Codes | Xi |
| Risk Statements | 36/38 |
| Safety Statements | 26 |
| RIDADR | UN 1993 / PGIII |
| WGK Germany | WGK 2 |
| Flash Point(F) | 188.6 °F - closed cup |
| Flash Point(C) | 87 °C - closed cup |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |
| Components | Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution |