Protease Fluorescent Detection Kit
SIGMA/PF0100 - high sensitivity assay
Synonym: Protease Assay Kit
Product Type: Chemical
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| Analysis Note: | Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases). |
| Application: | Fluorescent Protease Detection Kit Video Protocol |
| Application: | Detection limit: approx. 50 ng trypsin |
| Application: | Protease Fluorescent Detection Kit has been used: • in the detection of proteolytic activity using casein based protease assay • for testing the extracellular proteolytic activity of fungal strains • for quantifying mitochondrial protease activity • for measuring the protease production from soil solution |
| Preparation Note: | Supplied with sufficient reagents for up to 200 one mL assays. The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit. |
| Symbol |     GHS05,GHS07,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H314 - H334 - H335 - H410 |
| Precautionary statements | P261 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| Hazard Codes | C,N |
| Risk Statements | 35-50/53 |
| Safety Statements | 26-36/37/39-45-61 |
| RIDADR | UN 3316 9 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |
| Components | Incubation buffer 25 mL; FITC-casein solution 5 mL; Assay buffer 200 mL; Protease standard (trypsin) 20 μg; FITC control 50 mg; Trichloroacetic acid (TCA) solution 30 mL |