Isopropyl β-D-1-thiogalactopyranoside
SIGMA/PHG0010
Synonym: IPTG; Isopropyl β-D-thiogalactoside
CAS Number: 367-93-1
Empirical Formula (Hill Notation): C9H18O5S
Molecular Weight: 238.30
EC Number: 206-703-0
MDL Number: MFCD00063273
Linear Formula: C9H18O5S
Product Type: Chemical
IPTG induction mechanism illustration. Lac promoters are under the control of Lac repressor and is activated only in the presence of lactose or its synthetic analog IPTG. Like lactose, IPTG binds the repressor and releases his binding to the promoter, but unlike lactose, it′s not metabolized. The release of the repressor allows the transcription of the target gene.
| biological source | synthetic |
| form | powder |
| InChI | 1S/C9H18O5S/c1-4(2)15-9-8 |
| InChI key | BPHPUYQFMNQIOC-NXRLNHOXSA |
| Quality Level | 300  |
| SMILES string | CC(C)S[C@@H]1OC(CO)[C@H]( |
| storage temp. | 2-8°C |
| Application: | IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ. |
| General description: | Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program. M-Clarity Program Our comprehensive portfolio of upstream process chemicals not only provides biopharmaceutical manufacturers with high-quality raw materials for production of classical and novel therapies, but also helps them get to market faster and simplify regulatory challenges. Trust us to deliver supply chain transparency and reliable sourcing around the globe, streamlining your product qualification with best-in-class regulatory support and service. |
| Legal Information: | SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Non-metabolizable galactose analog. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352201 |