Inhibitor Resistant Genotyping PCR ReadyMix™
SIGMA/PIR00 - for Bio-Rad, Cepheid, Eppendorf, Illumina, Corbett, and Roche systems
Synonym: Inhibitor Resistant Genotyping PCR ReadyMix™
Product Type: Chemical
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| color | light blue |
| compatibility | Bio-Rad CFX384 |
| Bio-Rad CFX96 | |
| Bio-Rad MiniOpticon | |
| Bio-Rad MJ Chromo4 | |
| Bio-Rad MJ Opticon | |
| Bio-Rad MJ Opticon 2 | |
| Cepheid SmartCycler | |
| Eppendorf® Mastercycler ep realplex | |
| Eppendorf® Mastercycler ep realplex2 S | |
| Illumina Eco qPCR | |
| Qiagen Corbett Rotor-Gene 3000 | |
| Qiagen Corbett Rotor-Gene 6000 | |
| Qiagen Corbett Rotor-Gene Q | |
| Roche LightCycler 480 | |
| detection method | probe-based |
| feature | Difficult Templates/Specialty Enzymes PCR |
| dNTPs included | |
| hotstart | |
| form | liquid |
| input | crude DNA |
| Quality Level | 200  |
| shipped in | dry ice |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | qPCR: suitable |
| usage | sufficient for 1250 reactions |
| sufficient for 250 reactions | |
| sufficient for 5000 reactions |
| Application: | Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart® One-Step Probe RT-qPCR ReadyMix™ does not contain an internal reference dye. Inhibitor Resistant Genotyping PCR ReadyMix™ has been used as a component of the reaction mixture for detection of leucoanthocyanidin reductase(PaLAR3) alleles in a genotyping assay and multiplex PCR for amplification of microsatellite. |
| Features and Benefits: | • Assay results in as little as 33 minutes • Highly efficient and sensitive real-time PCR results • Little/no optimization required • Compatible with both fast and conventional PCR cycling protocols |
| General description: | Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use master mix for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product. A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification. |
| Legal Information: | Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | CFX384 is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | CFX96 is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | Chromo4 is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | Eppendorf is a registered trademark of Eppendorf AG |
| Legal Information: | KiCqStart is a registered trademark of QIAGEN Beverly Inc. |
| Legal Information: | LightCycler is a registered trademark of Roche |
| Legal Information: | Mastercycler is a registered trademark of Eppendorf AG |
| Legal Information: | MiniOpticon is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | Opticon is a trademark of Bio-Rad Laboratories, Inc. |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | Rotor-Gene is a registered trademark of Qiagen GmbH |
| Legal Information: | SmartCycler is a registered trademark of Cepheid, Inc. |
| Legal Information: | TaqMan is a registered trademark of Roche Molecular Systems, Inc. |
| Other Notes: | 2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers. packaging: 250 reactions* = 2 X 1.25 mL tubes 1250 reactions* = 10 X 1.25 mL tubes 5000 reactions* = 1 X 50 mL tube *number of reactions based on a 20uL volume |
| Other Notes: | Compatible instruments include: • Bio-Rad® CFX384™ • Bio-Rad CFX96™ • Bio-Rad MiniOpticon™ • Bio-Rad/MJ Chromo4™ • Bio-Rad/MJ Opticon 2 • Bio-Rad/MJ Opticon™ • Cepheid SmartCycler® • Eppendorf Mastercycler® ep realplex • Eppendorf Mastercycler ep realplex2 s • Illumina Eco qPCR • Qiagen/Corbett Rotor-Gene® 3000 • Qiagen/Corbett Rotor-Gene 6000 • Qiagen/Corbett Rotor-Gene Q • Roche LightCycler® 480 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106300 |