PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling

SIGMA/PKH67GL - Distributed for Phanos Technologies

Synonym: Green PKH membrane labeling kit

Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-PKH67GL-1KT	1 kit	$1030.00 1/EAAdd To Favorites		Add To Favorites

Internalization kinetics of PKH67 in L929 cells. (Photo courtesy of Cecile Rousselle and Dr. Xavier Ronot, Universite Joseph Fourier, Grenoble, France)

detection method	fluorometric
fluorescence	λ_ex 490 nm; λ_em 502 nm (PKH67 dye)
packaging	pkg of 1 kit
Quality Level	200
shipped in	ambient
storage condition	protect from light
storage temp.	room temp

Application:	PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling has been used: • To label and further investigate the exosomes expelled from cells in triple-negative breast cancer condition. • To label apoptotic cells, in order to study the role of ανβ5 receptor in both binding and internalization of apoptotic cells. • In fluorescence imaging.
Application:	This kit is for general cell membrane labeling. PKH67 has a longer aliphatic carbon tail than PKH1 and PKH2, two other green dyes previously described for in vitro and in vivo cell tracking. Based on the longer tail length, in-house studies have consistently shown reduced cell-cell transfer for PKH67 as compared to PKH2. Slow loss of fluorescence has been observed in in vivo studies using PKH1 and PKH2. PKH67 may exhibit similar properties since this behavior appears to be characteristic of green cell linker dyes, but not red cell linker dyes. Correlation of in vitro cell membrane retention with in vivo fluorescence half life in non-dividing cells predicts an in vivo fluorescence half life of 10-12 days for PKH67. Other green cell linker dyes with similar half lives have been used to monitor in vivo lymphocyte and macrophage trafficking over periods of 1-2 months, suggesting that PKH67 will also be useful for in vivo tracking studies of moderate length.
Legal Information:	CellVue is a registered trademark of Phanos Technologies
Linkage:	For additional technical details on PKH and CellVue^® Fluorescent Cell Linker Dyes including an extensive bibliography, please visit here .

Symbol	GHS02,GHS07
Signal word	Danger
Hazard statements	H225 - H319
Precautionary statements	P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
Hazard Codes	F
Risk Statements	11
Safety Statements	7-16
RIDADR	UN 3316 9
WGK Germany	WGK 3
Flash Point(F)	57.2 °F - closed cup
Flash Point(C)	14 °C - closed cup