Rabbit anti-c-myc Antibody, Affinity Purified
SIGMA/PLA0001 - Powered by Bethyl Laboratories, Inc.
Synonym: MRTL; MYCC; avian myelocytomatosis viral oncogene homolog; bHLHe39; c-Myc; class E basic helix-loop-helix protein 39; myc-related translation/
Product Type: Product-on-demand
Western Blotting Rabbit Anti-c-myc Antibody Affinity Purified: Cat. No. PLA0001: Detection of c-myc-tagged Protein by Western Blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody: Affinity purified Rabbit Anti-HA Antibody PLA0001 used for WB at 0.04 μg/mL (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
| accession no. | NP_002458.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 1:1,000- 1:30,000 |
| ELISA: 1:100- 1:500 (for coating plates) | |
| immunocytochemistry: 1:100-1:400 | |
| immunoprecipitation (IP): 1- 4 μg/mg | |
| western blot: 1:1,000- 1:30,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Rabbits were immunized with a synthetic peptide representing amino acid residues 410—419 (EQKLISEEDL) of human myc conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support. |
| Other Notes: | The c-myc epitope tag is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The recognized c-myc epitope represents amino acid residues 410-419, EQKLISEEDL, of human c-myc. |
| Physical form: | Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |