Goat anti-RENT1 Antibody, Affinity Purified
SIGMA/PLA0010 - Powered by Bethyl Laboratories, Inc.
Synonym: ATP-dependent helicase RENT1; HUPF1; KIAA0221; NORF1; RENT1; UP Frameshift 1; UPF1 regulator of nonsense transcripts homolog; UPF1 regulator of nonsense transcripts homolog (yeast); delta helicase; nonsense mRNA reducing factor 1; nonsense mediated mRNA decay factor; nonsense mediated mRNA decay factor (C. elegans); pNORF1; regulator of nonsense transcripts 1; smg-2; smg-2 homolog; smg-2 homolog; up-frameshift mutation 1 homolog; up-frameshift suppressor 1 homolog; yeast Upf1p homolog
Product Type: Product-on-demand
Western Blotting Goat Anti-RENT1 Antibody, Affinity Purified: Cat. No. PLA0010: Detection of Human RENT-1 by Western Blot. Samples: Whole cell lysate (30 μg) from lymphoblastoid cell lines (EBV-immotalized B-cell from a normal individual). Antibody: Affinity purified goat Anti-RENT-1 Antibody PLA0010 used at 1 μg/mL. Detection: Chemiluminescence.
| accession no. | NP_002902.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-10 μg/mL |
| western blot: 1:2,000-1:10000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0010 maps to a region between residue 1075 and the C-terminus (residue 1118) of human Regulator of Nonsense Transcripts 1 (up-frameshift mutation 1 homolog) using the numbering given in entry NP_002902.2 (GeneID 5976). |
| Other Notes: | RENT1, also known as UPF1, is a key regulator of the nonsense-mediated mRNA decay (NMD) pathway. The NMD pathway eliminates mRNAs that prematurely terminate during translation and may serve to protect the cells from dominant-negative effects of truncated proteins, or as a means to regulate protein expression. RENT1 is an ATP-dependent 5’- 3’ RNA helicase and ATPase that localizes to the cytoplasm and is part of a post-splicing multiprotein complex as well as an mRNA decay complex. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |