Rabbit anti-MDC1 Antibody, Affinity Purified
SIGMA/PLA0016 - Powered by Bethyl Laboratories, Inc.
Synonym: Em:AB023051.5; KIAA0170; NFBD1; homologue to Drosophila photoreceptor protein calphotin; mediator of DNA-damage checkpoint 1; nuclear factor with BRCT domains 1
Product Type: Product-on-demand
Western Blotting Rabbit Anti-MDC1 Antibody, Affinity Purified: Cat. No. PLA0016: Detection of Human MDC1 by Western Blot. Samples: Nuclear extract (50 μg) from HeLa cells. Antibody: Affinity purified Rabbit Anti-MDC1 antibody Cat. No. PLA0016 used at the indicated concentrations for WB. Detection: Chemiluminescence with exposure times less than 5 minutes.
| accession no. | NP_055456.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg/mg |
| western blot: 1:2000-1:10000 | |
| UniProt accession no. | Q14676  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0016 maps to a region between residues 475 and 525 of human Mediator of DNA Damage Checkpoint 1 using the numbering given in entry NP_055456.1 (GeneID 9656). |
| Other Notes: | MDC1 is required for the S- and G2/M-checkpoint-mediated cell cycle arrest in response to DNA damage. MDC1 interacts with H2AX near sites of DNA double-strand breaks and recruits ATM kinase to foci of DNA damage. MDC1 also appears to regulate the function of other proteins involved in the DNA damage checkpoint such as BRCA1 and Chk2. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |