Rabbit anti-SMC1 Antibody, Affinity Purified
SIGMA/PLA0017
Synonym: CDLS2; DXS423E; KIAA0178; SB1.8; SMC protein 1A; SMC-1-alpha; SMC-1A; SMC1; SMC1 (structural maintenance of chromosomes 1; yeast)-like 1; SMC1 structural maintenance of chromosomes 1-like 1 (yeast); SMC1L1; SMC1alpha; Smcb; segregation of mitotic chromosomes 1
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-SMC1 Antibody, Affinity Purified: Cat. No. PLA0017: Detection of Human SMC1 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-SMC1 (Cat. No. PLA0017) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-SMC1 Antibody, Affinity Purified: Cat. No. PLA0017: Detection of Human, Mouse and Xenopus SMC1 by Western Blot. Samples: A. Nuclear extracts from HeLa cells (50 μg) and Xenopus oocytes (100 μg). B. Whole cell lysate (100 μg) from MEF cells. Antibody: Affinity purified Rabbit Anti-SMC1 PLA0017 used at 1 μg/mL (A. Human), 2 μg/mL (A. Xenopus) or 0.5 μg/mL (B. Mouse). Detection: Chemiluminescence.
| accession no. | NP_006297.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:2,000-1:10,000 |
| immunoprecipitation (IP): 2-10 μg/mg | |
| western blot: 1:2,000-1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0017 maps to a region between residue 1175 and the C-terminus of human Structural Maintenance of Chromosomes 1 using the numbering given in entry NP_006297.2 (GeneID 8243). |
| Other Notes: | Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a heterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holds sister chromatids together. Cohesin is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |