Rabbit anti-SMC3 Antibody, Affinity Purified
SIGMA/PLA0019 - Powered by Bethyl Laboratories, Inc.
Synonym: BAM; BMH; CDLS3; CSPG6; Chondroitin sulfate proteoglycan 6; HCAP; SMC protein 3; SMC-3; SMC3L1; bamacan; bamacan proteoglycan; basement membrane-associated chondroitin proteoglycan; chondroitin sulfate proteoglycan 6 (bamacan); chromosome-
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-SMC3 Antibody, Affinity Purified: Cat. No. PLA0019: Detection of Human SMC3 by Immunohistochemistry. Sample: FFPE section of Human testicular seminoma. Antibody: Affinity purified Rabbit Anti-SMC3 (Cat. No. PLA0019) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-SMC3 Antibody, Affinity Purified: Cat. No. PLA0019: Detection of Human and Mouse SMC3 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, Jurkat, Mouse TCMK-1, and Mouse NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-SMC3 Antibody PLA0019 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunoprecipitation Rabbit Anti-SMC3 Antibody, Affinity Purified: Cat. No. PLA0019: Detection of Human SMC3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-SMC3 antibody Cat. No. PLA0019 used for IP at 6 μg per reaction. SMC3 was also immunoprecipitated by a previous lot of this antibody. For blotting immunoprecipitated SMC3, PLA0019 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
| accession no. | NP_005436.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:1,000-1:5,000 |
| immunoprecipitation (IP): 2-5 μg/mg | |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | Q9UQE7  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0019 maps to a region between residue 1175 and the C-terminus (residue 1217) of human Structural Maintenance of Chromosomes 3 using the numbering given in entry NP_005436.1 (GeneID 9126). |
| Other Notes: | Structural maintenance of chromosomes (SMC) proteins are part of the cohesin and condensin multisubunit complexes that play a critical role in chromosome organization, segregation, gene regulation, and DNA repair. The core cohesin complex includes a heterodimer of SMC1 and SMC3 that functions by forming a ring-like structure that holds sister chromatids together. Cohesin is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |