Rabbit anti-Rad21 Antibody, Affinity Purified
SIGMA/PLA0022 - Powered by Bethyl Laboratories, Inc.
Synonym: CDLS4; HR21; HRAD21; KIAA0078; MCD1; NXP-1; NXP1; RAD21 (S. pombe) homolog; RAD21 homolog; RAD21 homolog (S. pombe); SCC1; SCC1 homolog; hHR21; kleisin; nuclear matrix protein 1; protein involved in DNA double-strand break repair; sister chromatid cohesion 1
Product Type: Product-on-demand
Western Blotting Rabbit Anti-Rad21 Antibody, Affinity Purified: Cat. No. PLA0022: Detection of Human and Mouse Rad21 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg), 293T (Lane T; 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-Rad21 Antibody PLA0022 used for WB at 0.2 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
| accession no. | NP_006256.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-10 μg/mg |
| western blot: 1:2,000-1:10,000 | |
| UniProt accession no. | O60216  |
| Biochem/physiol Actions: | RAD21 (RAD21 homolog, S. pombe) plays a crucial role in normal cell division. It acts as a main component of the cohesin complex for the proper chromosomal arrangement during cell division. The protein possesses two mitotic cleavage sites for the attachment of separase. It has been shown experimentally that at the beginning of anaphase stage, a caspase-like Esp1/separase attaches to the cleavage site of RAD21 and initiate sister chromatid separation, which is an essential step for the completion of cytokinesis. During double strand DNA damage repair, it helps the two DNA strands to adhere with each other. Mutation in RAD21 causes a congenital phenotype, cohesinopathy in humans characterized with growth retardation and minor skeletal anomalies. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | RAD21 (RAD21 homolog, S. pombe) is a novel nuclear protein located on chromosome 8q24. |
| Immunogen: | The epitope recognized by PLA0022 maps to a region between residue 575 and the C-terminus (residue 631) human Rad21 homolog using the numbering given in entry NP_006256.1 (GeneID 5885). |
| Other Notes: | Rad21, also called SCC1 (sister chromatid cohesion protein 1), is part of the mitotic cohesin complex that consist of SMC1, SMC3, SCC3 and SCC1. The cohesin complex is important to sister chromatid separation and segregation during mitosis and meiosis and also plays a role in double-strand break repair by homologous recombination. Rad21 has been demonstrated to be cleaved by caspase proteins to trigger sister chromatid separation and during apoptotic signaling. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |