Rabbit anti-H2AX Antibody, Affinity Purified
SIGMA/PLA0023 - Powered by Bethyl Laboratories, Inc.
Synonym: H2A histone family; H2A.X; H2A/X; H2AX; H2AX histone; histone H2A.x; member X
Product Type: Product-on-demand
Western Blotting Rabbit Anti-H2AX Antibody, Affinity Purified: Cat. No. PLA0023: Detection of Human and Mouse Histone H2AX by Western Blot. Samples: Nuclear extract from Human 293T, Human G-361, and wild-type (+/+) or H2AX knockout (-/-) Mouse embryonic fibroblasts. Antibody: Affinity purified Rabbit Anti-H2AX Antibody PLA0023 used at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 5 seconds.
| accession no. | P16104 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500-1:2,000 |
| western blot: 1:2,000-1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recongized by PLA0023 maps to the C-terminus of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014). |
| Other Notes: | H2AX is a member of the histone H2A family. The four core histones involved in the formation of the nucleosome structure of compacted chromatin are H2A, H2B, H3, and H4. H2AX may function to facilitate DNA repair, and recent studies have shown that H2AX is required for the maintenance of genomic stability. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |