Goat anti-Menin Antibody, Affinity Purified
SIGMA/PLA0027
Synonym: MEAI; SCG2; menin; multiple endocrine neoplasia I
Product Type: Product-on-demand
Western Blotting Goat Anti-Menin Antibody, Affinity Purified: Cat. No. PLA0027: Detection of Human Menin by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Goat Anti-Menin antibody Cat. No. PLA0027 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
| accession no. | NP_000235.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-10 μg/mg |
| western blot: 1:1,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0027 maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221). |
| Other Notes: | Menin is the gene product of the putative tumor suppressor MEN1 associated with the syndrome termed multiple endocrine neoplasia type 1 that may function in the regulation of DNA synthesis or the response to DNA damage. Menin has been shown to interact with JunD, a member of the AP-1 heterodimer and inhibit transcription. Menin has also been shown to interact with Pem, FANCD2, RPA, NMMHCII-A, GFAP, vimentin, Hsp70, NfkappaB proteins, Smads, mSin3A and is part of the MLL2 and MLL1 Set1-like histone H3 K4 methyltransferase complex where it interacts directly with ASH2, Rbbp5, and WDR5. As part of the MLL complexes, menin may mediate its tumor suppressor activity via the regulation of histone methylation of HOX and CDK inhibitor genes. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |