Rabbit anti-TopBP1 Antibody, Affinity Purified
SIGMA/PLA0030 - Powered by Bethyl Laboratories, Inc.
Synonym: DNA topoisomerase II-beta-binding protein 1; DNA topoisomerase II-binding protein 1; KIAA0259; TOP2BP1; TopBP1
Product Type: Product-on-demand
Western Blotting Rabbit Anti-TopBP1 Antibody, Affinity Purified: Cat. No. PLA0030: Detection of Human TOPBP1 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit Anti-TOPBP1 antibody Cat. No. PLA0030 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blotting Rabbit Anti-TopBP1 Antibody, Affinity Purified: Cat. No. PLA0030: Detection of Mouse TopBP1 by Western Blot. Samples: Whole cell lysate from TCMK-1 (15 and 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-TopBP1 Antibody PLA0030 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
| accession no. | NP_008958.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg/mg |
| western blot: 1:2,000-1:10,000 | |
| UniProt accession no. | Q92547  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0030 maps to a region between residue 1400 and the C-terminus (residue 1435) of human Topoisomerase (DNA) II Binding Protein 1 using the numbering given in entry NP_008958.1 (GeneID 11073). |
| Other Notes: | Topoisomerases regulate topological states of DNA. DNA topoisomerase II-binding protein 1 (TopBP1) is a protein that interacts with the C-terminal region of topoisomerase II beta (Topo II beta). TopBP1 function has been found to be important to various aspects of cellular growth control such as DNA replication, the DNA damage checkpoint, and cell survival. TopBP1 interacts with several proteins through its BRCA1 C-terminal (BRCT) motifs. TopBP1 interacts and inhibits E2F1 transcriptional activity through an Rb-independent mechanism that involves recruitment of the SWI/SNF chromatin-remodeling complex proteins Brg1 and Brm. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |