Goat anti-MCM4 Antibody, Affinity Purified
SIGMA/PLA0039 - Powered by Bethyl Laboratories, Inc.
Synonym: CDC21; CDC21 homolog; CDC54; MCM4 minichromosome maintenance deficient 4 (S. cerevisiae); MGC33310; NKCD; NKGCD; P1-Cdc21; hCdc21; homolog of S. pombe cell devision cycle 21; minichromosome maintenance deficient 4
Product Type: Product-on-demand
Immunohistochemistry Goat Anti-MCM4 Antibody, Affinity Purified: Cat. No. PLA0039: Detection of Human MCM4 by Immunohistochemistry. Sample: FFPE section of Human ovarian carcinoma. Antibody: Affinity purified goat Anti-MCM4 (Cat. No. PLA0039) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Goat Anti-MCM4 Antibody, Affinity Purified: Cat. No. PLA0039: Detection of Human MCM4 by Western Blot. Sample: Whole cell lysate (60 μg) from HeLa cells. Antibody: Affinity purified goat Anti-MCM4 PLA0039 used at 0.25 μg/mL. Detection: Chemiluminescence.
| accession no. | NP_005905.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500-1:2,000 |
| western blot: 1:2,000-1:10,000 | |
| UniProt accession no. | P33991  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0039 maps to a region between residues 325 and 375 of human Minichromosome Maintenance 4 using the numbering given in entry NP_005905.2 (GeneID 4173). |
| Other Notes: | The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM4 is part of the core MCM complex that includes MCM4, MCM6, and MCM7. The core complex possesses DNA helicase activity. MCM4 is phosphorylated by CDC2 kinase resulting in a reduction of helicase activity and chromatin binding. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |