Rabbit anti-ATR Antibody, Affinity Purified
SIGMA/PLA0046 - Powered by Bethyl Laboratories, Inc.
Synonym: FCTCS; FRAP-related protein 1; FRAP-related protein-1; FRP1; MEC1; MEC1; MEC1; SCKL; SCKL1; ataxia telangiectasia and Rad3 related; ataxia telangiectasia and Rad3-related protein; homolog; homolog (S. cerevisiae); mitosis entry checkpoint 1; mitosis entry checkpoint 1
Product Type: Product-on-demand
Western Blotting Rabbit Anti-ATR Antibody, Affinity Purified: Cat. No. PLA0046: Detection of Human ATR by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB and 293T (Lane T; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-ATR antibody Cat. No. PLA0046 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
Immunoprecipitation Rabbit Anti-ATR Antibody, Affinity Purified: Cat. No. PLA0046: Detection of Human ATR by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-ATR antibody Cat. No. PLA0046 used for WB at 0.1 μg/mL and IP at 3 μg/mg lysate. ATR was also immunoprecipitated by ATR Antibody B. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | NP_001175.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2- 5 μg/mg |
| western blot: 1:2,000-1:10,000 | |
| UniProt accession no. | Q13535  |
| Biochem/physiol Actions: | Ataxia-telangiectasia and Rad3-related protein (ATR) plays a key role in maintaining the integrity of the genome. It participates in stabilizing replication forks and modulates the cell cycle progression and DNA repair. ATR plays a vital role in cell viability. It sends out signals to control replication origin firing, replication fork repair, and mitotic entry prematurely. Lack of ATR affects normal development, aging, and tissue homeostasis. Mutation in the ATR gene is associated with Seckel syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Ataxia telangiectasia and Rad3-related protein (ATR), a serine/threonine kinase, is a member of the phosphoinositide 3- kinase-related protein kinase s (PIKKs) family mainly to ataxia telangiectasia mutated (ATM) subfamily. It has a phosphatidylinositol 3/4 (PI3/4) kinase catalytic domain, 1 focal adhesion kinase, targeting (FAT) domain, 1 FATC domain, 1 UME domain, and 2 HEAT repeats. ATR has ATR-201 and ATR-202 isoforms. Isoform 1 is expressed at a high level in the testis. Isoform 2 is expressed in the pancreas, liver and, placenta. The ATR gene is located on human chromosome 3q23. |
| Immunogen: | The epitope recognized by PLA0046 maps to a region between residues 1675 and 1725 of human Ataxia Telangiectasia and Rad3-related using the numbering given in entry NP_001175.1 (GeneID 545). |
| Other Notes: | ATR (ATM and Rad3 related) is closely related to ATM (Ataxia telangiectasia, mutated) and is a member of the phosphatidylinositol 3 kinase (PI-3) family that is an early sensor of DNA damage. ATR is a serine-threonine kinase that reacts to UV damage and interruptions in replication. ATR may be able to sense DNA damage through interaction with Rad17 and 1as well as components of nucleosome remodeling complexes. In response to DNA damage, ATR has been shown to phosphorylate a multitude of substrates which include BRCA1, p53, Chk2, Rad 17, and E2F transcription factor 1. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |