Goat anti-Chk1 Antibody, Affinity Purified
SIGMA/PLA0054
Synonym: 1; CHK1; CHK1 (checkpoint; S.pombe) homolog; CHK1 checkpoint homolog; CHK1 checkpoint homolog (S. pombe); Checkpoint; Chk1-S; S. pombe; cell cycle checkpoint kinase; checkpoint kinase-1; homolog of
Product Type: Product-on-demand
Western Blotting Goat Anti-Chk1 Antibody, Affinity Purified: Cat. No. PLA0054: Detection of Human Chk1 by Western Blot. Samples: Whole cell lysate (30 μg) from HeLa cells treated with 10 Gy ionizing radiation (IR) or untreated. Antibody: Affinity purified goat Anti-Chk1 (Cat. No. PLA0054) used at 0.1 μg/mL. Detection: Chemiluminescence with 30 second exposure.
| accession no. | NP_001265.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:2,500- 1:15,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0054 maps to a region between residues 200 and 250 of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111). |
| Other Notes: | Chk1 functions as a checkpoint protein kinase that is critical to the regulation of the Cdc25a and Cdc25c phosphatases that promote S and G2/M phase progression, respectively. Chk1 kinase activity inhibits cell cycle progression in the presence of DNA damage by phosphorylating Cdc25a and Cdc25c and causing the degradation of Cdc25a and cytoplasmic sequestration of Cdc25c. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |