Rabbit anti-MCM2 Antibody, Affinity Purified
SIGMA/PLA0060 - Powered by Bethyl Laboratories, Inc.
Synonym: BM28; CCNL1; CDCL1; D3S3194; DFNA70; KIAA0030; MCM2 minichromosome maintenance deficient 2; cdc19; cell devision cycle-like 1; cyclin-like 1; minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin); minichromosome maintenance deficient 2 (mitotin); minichromosome maintenance protein 2 homolog; mitotin; mitotin (S. cerevisiae); nuclear protein BM28
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-MCM2 Antibody, Affinity Purified: Cat. No. PLA0060: Detection of Human MCM2 by Immunohistochemistry. Sample: FFPE section of Human small cell lung cancer. Antibody: Affinity purified Rabbit Anti-MCM2 (Cat. No. PLA0060) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-MCM2 Antibody, Affinity Purified: Cat. No. PLA0060: Detection of Human MCM2 by Western Blot and Immunoprecipitation. Samples: RIPA extract (50 μg for WB; 7 mg for CoIP) from HeLa cells. Antibody: Affinity purified Rabbit Anti-MCM2 (Cat. No. PLA0060) used at 0.2 μg/mL for WB and 20 μg/7 mg extract for IP. Detection: Chemiluminescence for WB; Coomassie staining for CoIP. Identity of CoIP proteins determined by mass spectrometry.
Western Blotting Rabbit Anti-MCM2 Antibody, Affinity Purified: Cat. No. PLA0060: Detection of Mouse MCM2 by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified Rabbit Anti-MCM2 Antibody PLA0060 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
| accession no. | NP_0045117.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:2,000-1:10,000 |
| immunoprecipitation (IP): 2- 5 μg/mg | |
| western blot: 1:1,000- 1:10,000 | |
| UniProt accession no. | P49736  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0060 maps to a region between residues 1 and 50 of human human minichromosomal maintenance deficient 2 using the numbering given in entry NP_0045117.2 (GeneID 4171). |
| Other Notes: | The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM2 interacts with the core MCM complex (MCM4, 6, and 7) to regulate its helicase activity. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |