Rabbit anti-RPA32 Antibody, Affinity Purified
SIGMA/PLA0070 - Powered by Bethyl Laboratories, Inc.
Synonym: 32kDa; REPA2; RF-A protein 2; RP-A p32; RP-A p34; RPA32; replication factor A protein 2; replication protein A 34 kDa subunit; replication protein A2; replication protein A2 (32kD)
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-RPA32 Antibody, Affinity Purified: Cat. No. PLA0070: Detection of Human RPA32 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-RPA32 (Cat. No. PLA0070) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-RPA32 Antibody, Affinity Purified: Cat. No. PLA0070: Detection of Human and Mouse RPA32 by Western Blot. Samples: Whole cell lysate (50 μg) from 293T, HeLa, Jurkat, Mouse TCMK-1, and Mouse NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-RPA32 Antibody PLA0070 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunoprecipitation Rabbit Anti-RPA32 Antibody, Affinity Purified: Cat. No. PLA0070: Detection of Human RPA32 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from 293T cells. Antibodies: Affinity purified Rabbit Anti-RPA32 antibody Cat. No. PLA0070 used for IP at 6 μg/mg lysate. RPA32 was inefficiently immunoprecipitated by Rabbit Anti-RPA32 antibody B. For blotting immunoprecipitated RPA32, PLA0070 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
| accession no. | NP_002937.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:2,000- 1:10,000 |
| immunoprecipitation (IP): 2-5 μg/mg | |
| western blot: 1:2,000-1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0070 maps to a region between residue 225 and the C-terminus (residue 270) of human Replication Protein A2 (32kD) using the numbering given in entry NP_002937.1 (GeneID 6118). |
| Other Notes: | Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |