Rabbit anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified
SIGMA/PLA0071 - Powered by Bethyl Laboratories, Inc.
Synonym: 32kDa; REPA2; RF-A protein 2; RP-A p32; RP-A p34; RPA32; replication factor A protein 2; replication protein A 34 kDa subunit; replication protein A2; replication protein A2 (32kD)
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified: Cat. No. PLA0071: Detection of Human and Mouse Phospho RPA32 (S4/S8) by Immunohistochemistry. Sample: FFPE sections of Human ovarian carcinoma (left) and Mouse renal cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-Phospho RPA32 (S4/S8) (Cat. No. PLA0071) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified: Cat. No. PLA0071: Detection of Human Phospho RPA32 (S4/S8) by Western Blot. Samples: Whole cell lysate (50 μg) from 293T cells treated with either 100 μM etoposide for 2 hours (+) or mock treated (-) cells. Antibodies: Affinity purified Rabbit Anti-Phospho RPA32 (S4/S8) Antibody PLA0071 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower panel shows Westen Blot for total RPA32 using affinity purified Rabbit Anti-RPA32 Antibody PLA0070 at 1 μg/mL with an exposure time of 3 seconds.
Immunoprecipitation Rabbit Anti-Phospho RPA32 (S4/S8) Antibody, Affinity Purified: Cat. No. PLA0071: Detection of Human Phospho RPA32 (S4/S8) by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells treated with either 100 μM etoposide for 2 hours (+) or mock treated (-) cells. Antibodies: RPA32 was immunoprecipated using Rabbit Anti-RPA32 antibody Cat. No. PLA0070. Following immunoprecipitation, the product of the IP reaction was either mock treated (-) or treated with phosphatases (+). Affinity purified Rabbit Anti-phospho RPA32 (S4/S8) antibody Cat. No. PLA0071 was used for WB at 1 mcg/mL. To examine total RPA32, the blot was stripped and probed with Rabbit Anti-RPA32 antibody PLA0070. Detection: Chemiluminescence with an exposure time of 3 minutes (upper panel) and 3 seconds (lower panel).
| accession no. | NP_002937.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | phosphorylation (pSer4/pSer8) |
| technique(s) | immunocytochemistry: 1:500-1:5,000 |
| immunohistochemistry: 1:500-1:2,000 | |
| immunoprecipitation (IP): 2-10 μg/mg | |
| western blot: 1:2,000-1:10,000 |
| Biochem/physiol Actions: | Replication Protein A (RPA) binds single-stranded DNA and plays a vital role in DNA replication, DNA repair, and checkpoint signaling. Phosphorylation of replication protein A2 (RPA32) by ataxia telangiectasia and Rad3-related protein kinase (ATR) and DNA-dependent protein kinase (DNA-PK) causes checkpoint kinase 1 (Chk1) activation and replication arrest. DNA-PK and ATR signaling via RPA32 (S4/S8) enhances genome stability and cell survival following replication stress. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Replication protein A2 (RPA32) is encoded by the gene mapped to human chromosome 1p35.3. It is a subunit of the heterotrimeric replication protein A (RPA) complex. |
| Immunogen: | Immunogen was a dually phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serines that corresponded to positions 4 and 8 using the numbering given in entry NP_002937.1 (GeneID 6118). |
| Other Notes: | Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |