Rabbit anti-BARD1 Antibody, Affinity Purified
SIGMA/PLA0074 - Powered by Bethyl Laboratories, Inc.
Synonym: BARD-1; BRCA1-associated RING domain gene 1
Product Type: Product-on-demand
Western Blotting Rabbit Anti-BARD1 Antibody, Affinity Purified: Cat. No. PLA0074: Detection of Human BARD1 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified Rabbit Anti-BARD1 Antibody PLA0074 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation Rabbit Anti-BARD1 Antibody, Affinity Purified: Cat. No. PLA0074: Detection of Human BARD1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BARD1 antibody Cat. No. PLA0074 used for IP at 6 μg/mg lysate. BARD1 was also immunoprecipitated by BARD1 Antibody B. For blotting immunoprecipitated BARD1, PLA0074 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
| accession no. | NP_000456.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-10 μg/mg |
| western blot: 1:500-1:2,500 | |
| UniProt accession no. | Q99728  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0074 maps to a region between residues 250 and 300 of human BRCA1-associated RING Domain 1 using the numbering given in entry NP_000456.1 (GeneID 580). |
| Other Notes: | BRCA1 associated RING domain 1 (BARD1) together with BRCA1 is an E3 ubiquitin ligase. BARD1/BRCA1 has been shown to ubiquinate RNA polymerase II in response to DNA damage. BARD1/BRCA1 ubiquitin ligase activity has also been shown to be important in the prevention of double stranded breaks and the regulation of centrosome number. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |