Rabbit anti-53BP1 Antibody, Affinity Purified
SIGMA/PLA0077 - Powered by Bethyl Laboratories, Inc.
Synonym: 1; 1; 53BP1; TDRD30; TP53; p202; p53-binding protein 1; p53BP1; tumor protein 53-binding protein; tumor protein p53-binding protein
Product Type: Product-on-demand
Western Blotting Rabbit Anti-53BP1 Antibody, Affinity Purified: Cat. No. PLA0077: Detection of Human [deleted Mouse] 53BP1 by Western Blot Sample: Whole cell lysate (20 μg/lane) from U2OS cells resolved on a 3 to 8% tris-acetate gel. Antibody: Affinity purified Rabbit Anti-53BP1 (Cat. No. PLA0077) used at 0.17 μg/mL. Detection: Chemiluminescence with a 15 second exposure.
| accession no. | NP_005648.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:2,000-1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0077 maps to a region between residues 1925 and the C-terminus (residue 1972) of human tumor protein p53 binding protein 1 using the numbering given in entry NP_005648.1 (GeneID 7158). |
| Other Notes: | 53BP1 (p53 Binding Protein 1) is an early participant in the cellular response to DNA double-strand breaks. 53BP1 functions in a redundant role in phosphorylation of the downstream checkpoint effector proteins Brca1 and Chk2 but was required for the formation of Brca1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |