Rabbit anti-KAP-1 Antibody, Affinity Purified
SIGMA/PLA0078 - Powered by Bethyl Laboratories, Inc.
Synonym: E3 SUMO-protein ligase TRIM28; KAP-1; KAP1; KRAB [Kruppel-associated box domain]-associated protein 1; KRAB-associated protein 1; KRAB-interacting protein 1; KRIP-1; PPP1R157; RING finger protein 96; RNF96; TF1B; TIF1-beta; TIF1B; Tripartite motif-containing protein 28; nuclear corepressor KAP-1; protein phosphatase 1; regulatory subunit 157; transcriptional intermediary factor 1-beta
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-KAP-1 Antibody, Affinity Purified: Cat. No. PLA0078: Detection of Human KAP-1 by Immunohistochemistry and Immunocytochemistry. Sample: FFPE section of Human prostate carcinoma (left) and formaldelyde-fixed HeLa cells (right). Antibody: Affinity purified Rabbit Anti-KAP-1 (Cat. No.PLA0078) used at a dilution of 1:1,000 (1 μg/mL).Detection: DAB (left) and green fluorescent goat Anti-Rabbit IgG highly cross-adsorbed Antibody FITC conjugated used at a dilution of 1:100 (right).
Western Blotting Rabbit Anti-KAP-1 Antibody, Affinity Purified: Cat. No. PLA0078: Detection of Human KAP1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 μg), and 293T (50 μg) cells. Antibodies: Affinity purified Rabbit Anti-KAP1 Antibody PLA0078 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
Western Blotting Rabbit Anti-KAP-1 Antibody, Affinity Purified: Cat. No. PLA0078: Detection of Mouse KAP1 by Western Blot. Samples: Whole cell lysate from TCMK-1 (50 μg) cells. Antibodies: Affinity purified Rabbit Anti-KAP1 Antibody PLA0078 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
| accession no. | NP_005753.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 1:500-1:2,500 |
| immunohistochemistry: 1:500-1:2,000 | |
| immunoprecipitation (IP): 2-10 μg/mg | |
| western blot: 1:2,000-1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0078 maps to a region between residue 1 and 50 of human Tripartite Motif-Containing 28 using the numbering given in entry NP_005753.1 (GeneID 10155). |
| Other Notes: | KAP-1 (KRAB-Associated Protein 1) is a member of the tripartite motif RBCC/Trim domain family that contains a RING finger domain, B boxes, and an alpha helical coiled coil region. KAP-1 functions as a corepressor by interacting with the KRAB domain of KRAB zinc-finger transcriptional repressors. KAP-1 also appears to function as a scaffold for chromatin-remodeling complexes involved in transcriptional repression. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |