Rabbit anti-HIF1-alpha Antibody, Affinity Purified
SIGMA/PLA0081 - Powered by Bethyl Laboratories, Inc.
Synonym: ARNT interacting protein; ARNT-interacting protein; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PAS domain-containing protein 8; PASD8; alpha subunit (basic helix-loop-helix transcription factor); alpha subunit (basic helix-loop-helix transcription factor); bHLHe78; basic-
Product Type: Product-on-demand
Immunocytochemistry Rabbit Anti-HIF1-alpha Antibody, Affinity Purified: Cat. No. PLA0081: Detection of Human HIF1 alpha by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells treated with cobalt chloride-200 mcM (left) or mock treatment (right). Antibody: Affinity purified Rabbit Anti-HIF1 alpha (Cat. No. PLA0081) used at a dilution of 1:100 (2 μg/mL). Detection: Red-fluorescent Goat Anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated.
Western Blotting Rabbit Anti-HIF1-alpha Antibody, Affinity Purified: Cat. No. PLA0081: Detection of Human HIF1 alpha by Western Blot. Samples: Whole cell lysate (5, 15 and 50 μg for WB) from HeLa cells that were either treated with cobalt chloride (+; 200 mcM) or mock treated (-). Antibodies: Affinity purified Rabbit Anti-HIF1 alpha antibody Cat. No. PLA0081 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
Immunoprecipitation Rabbit Anti-HIF1-alpha Antibody, Affinity Purified: Cat. No. PLA0081: Detection of Human HIF1 alpha by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells that were either treated with cobalt chloride (+; 200 mcM) or mock treated (-). Antibodies: Affinity purified Rabbit Anti-HIF1 alpha antibody Cat. No. PLA0081 used for WB at 1 μg/mL and IP at 3 μg/mg lysate. HIF1 alpha was also immunoprecipitated by HIF1 Alpha Antibody B. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | Q16665 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: 1:100-1:500 |
| immunohistochemistry: 1:100-1:500 | |
| immunoprecipitation (IP): 2-10 μg/mg | |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0081 maps to a region between residues 775 and the C-terminus (residue 826) of human hypoxia-inducible factor 1, alpha subunit using the numbering given in Swiss-Prot entry Q16665 (GeneID 3091). |
| Other Notes: | Hypoxia-inducible factor-1 (HIF1) is a basic helix-loop-helix (bHLH)-PER-ARNT-sim (PAS) transcription factor that plays a role in the cellular response to hypoxia. HIF1 is a heterodimer of an HIF1-alpha and HIF1-beta subunit. During normoxia, HIF-1alpha is regulated by ubiquitination and interaction with the von-Hippel-Lindau tumor suppressor protein (pVHL). |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |