Rabbit anti-FUS Antibody, Affinity Purified
SIGMA/PLA0083 - Powered by Bethyl Laboratories, Inc.
Synonym: 75 kDa DNA-pairing protein; ALS6; ETM4; FUS1; HNRNPP2; POMP75; TLS; amyotrophic lateral sclerosis 6; derived from t(12;16) malignant liposarcoma; fus-like protein; fused in sarcoma; fusion; fusion (involved in t(12;16) in malignant liposarcoma); fusion gene in myxoid liposarcoma; heterogeneous nuclear ribonucleoprotein P2; hnRNP-P2; oncogene FUS; oncogene TLS; translocated in liposarcoma; translocated in liposarcoma protein
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-FUS Antibody, Affinity Purified: Cat. No. PLA0083: Detection of Human and Mouse FUS by Immunohistochemistry. Sample: FFPE section of Human ovarian carcinoma (left) and Mouse teratoma (right). Antibody: Affinity purified Rabbit Anti-FUS (Cat. No. PLA0083) used at a dilution of 1:5,000 (0.2 μg/mL) and 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-FUS Antibody, Affinity Purified: Cat. No. PLA0083: Detection of Human FUS by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 μg), 293T (Lane T; 50 μg) and Jurkat (Lane J; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-FUS antibody Cat. No. PLA0082 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with exposure time of 3 seconds.
Western Blotting Rabbit Anti-FUS Antibody, Affinity Purified: Cat. No. PLA0083: Detection of Mouse FUS by Western Blot. Samples: Whole cell lysate (50 μg) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified Rabbit Anti-FUS Antibody PLA0083 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunoprecipitation Rabbit Anti-FUS Antibody, Affinity Purified: Cat. No. PLA0083: Detection of Human FUS by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-FUS antibody Cat. No. PLA0083 used for WB at 0.1 μg/mL IP at 6 μg/mg lysate. FUS was also immunoprecipitated by FUS Antibody B. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | P35637 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:1,000-1:5,000 |
| immunoprecipitation (IP): 2-10 μg/mg | |
| western blot: 1:2,000-1:10,000 | |
| UniProt accession no. | P35637  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0083 maps to a region between residues 400 and 450 of human fusion (involved in t(12;16) in malignant liposarcoma) using the numbering given in SwissProt entry P35637 (GeneID 2521). |
| Other Notes: | FUS has been identified as a frequent translocation fusion partner of various transcription factors. FUS fusion genes have been shown to be associated with multiple tumor types which include liposarcoma, leukemia, histocytoma, and sarcoma. FUS is a multifunctional RNA-binding protein that associates with the nuclear matrix and Cajal bodies and appears to play a role in splicesome assembly, pre-mRNA splicing, DNA repair, transcriptional regulation and homologous recombination. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |