Rabbit anti-MAD2 Antibody, Affinity Purified
SIGMA/PLA0087 - Powered by Bethyl Laboratories, Inc.
Synonym: HSMAD2; MAD2; MAD2 (mitotic arrest deficient; MAD2-like protein 1; homolog)-like 1; homolog-like 1; mitotic arrest deficient; mitotic arrest deficient 2-like protein 1; yeast; yeast
Product Type: Product-on-demand
Western Blotting Rabbit Anti-MAD2 Antibody, Affinity Purified: Cat. No. PLA0087: Detection of Human and Mouse MAD2 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, Jurkat, Mouse TCMK-1, and Mouse NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-MAD2 antibody Cat. No. PLA0087 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation Rabbit Anti-MAD2 Antibody, Affinity Purified: Cat. No. PLA0087: Detection of Human MAD2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-MAD2 antibody Cat. No. PLA0087 used for IP at 6 μg/mg lysate. MAD2 was also immunoprecipitated by Rabbit Anti-MAD2 Antibody B. For blotting immunoprecipitated MAD2, Cat. No. PLA0087 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
| accession no. | Q13257 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-10 μg/mg |
| western blot: 1:2,000-1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0087 maps to a region between residue 150 and the C-terminus (residue 205) of human Mitotic Arrest Deficient 2 using the numbering given in SwissProt entry Q13257 (GeneID 4085). |
| Other Notes: | MAD2 (Mitotic Arrest Deficiency 2) is a component of the mitotic spindle-assembly checkpoint that ensures that all chromosomes are aligned at the metaphase plate before the onset of anaphase. MAD2 is a subunit of the MCC complex that includes BubR1, Bub3, Cdc20 and Mad2, which may function to inhibit the APC/c (Anaphase Promoting Complex/Cyclosome). MAD2 is regulated by MAD1. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |