Rabbit anti-MAD1 Antibody, Affinity Purified
SIGMA/PLA0092 - Powered by Bethyl Laboratories, Inc.
Synonym: HsMAD1; MAD1; MAD1 (mitotic arrest deficient; yeast; homolog)-like 1; MAD1 mitotic arrest deficient-like 1; MAD1-like protein 1; PIG9; TP53I9; TXBP181; hMAD1; homolog-like 1; mitotic arrest deficient 1-like protein 1; mitotic checkpoint MAD1 protein homolog; mitotic-arrest deficient 1; tax-binding protein 181; tumor protein p53 inducible protein 9; yeast
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-MAD1 Antibody, Affinity Purified: Cat. No. PLA0092: Detection of Human MAD1 by Immunohistochemistry. Sample: FFPE section of Human prostate carcinoma. Antibody: Affinity purified Rabbit Anti-MAD1 (Cat. No. PLA0092) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-MAD1 Antibody, Affinity Purified: Cat. No. PLA0092: Detection of Human MAD1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (50 μg/lane in A; 50 μg input in B, 1 mg for IP in B) from HeLa cells. In A, HeLa cells were treated with Nocodazole (+) or mock treated (-). Antibody: Affinity purified Rabbit Anti-MAD1 Antibody (Cat. No. PLA0092) used at 0.1 μg/mL for Western Blot (A) and at 1 μg/mg lysate for IP (B). MAD1 was also immunoprecipitated using Rabbit Anti-MAD1 Antibodies recognizing different epitopes using 1 μg/mg lysate. In B, MAD1 in input and immunoprecipitate was detected using Antibody at 0.2 μg/mL. Detection: Chemiluminescence with exposure times of 10 minutes (A) and 5 minutes (B).
| accession no. | Q9Y6D9 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500- 1:2,000 |
| immunoprecipitation (IP): 1-4 μg/mg | |
| western blot: 1:5,000- 1:15,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0092 maps to a region between residues 1 and 50 of human Mitotic arrest deficient-like 1 using the numbering given in Swiss-Prot entry Q9Y6D9 (GeneID 8379). |
| Other Notes: | MAD1 (Mitotic Arrest Deficiency 1) is a component of the mitotic spindle-assembly checkpoint that ensures that all chromosomes are aligned at the metaphase plate before the onset of anaphase. MAD1 functions as a homodimer that interacts with and regulates MAD2. MAD1 may play a role in tumor suppression. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |