Rabbit anti-p300 Antibody, Affinity Purified
SIGMA/PLA0096 - Powered by Bethyl Laboratories, Inc.
Synonym: 300kD; E1A-associated protein p300; E1A-binding protein; KAT3B; RSTS2; histone acetyltransferase p300; p300; p300 HAT
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-p300 Antibody, Affinity Purified: Cat. No. PLA0096: Detection of Human p300 by Immunohistochemistry. Sample: FFPE section of Human lung carcinoma. Antibody: Affinity purified Rabbit Anti-p300 (Cat. No. PLA0096) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-p300 Antibody, Affinity Purified: Cat. No. PLA0096: Detection of Human p300 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified Rabbit Anti-p300 Antibody PLA0096 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunoprecipitation Rabbit Anti-p300 Antibody, Affinity Purified: Cat. No. PLA0096: Detection of Human p300 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-p300 antibody Cat. No. PLA0096 used for IP at 6 μg/mg lysate. p300 was also immunoprecipitated by p300 Antibody B. For blotting immunoprecipitated p300, PLA0096 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 1 second.
| accession no. | NP_001420.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | ChIP: 10 μg |
| immunohistochemistry: 1:1,000- 1:5,000 | |
| immunoprecipitation (IP): 1-5 μg/mg | |
| western blot: 1:2,000-1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0096 maps to a region between residues 950 and 1000 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033). |
| Other Notes: | By sequence similarity p300 is related to CBP (CREB-binding protein) and functions as a histone acetyltransferase that can regulate transcription by influencing chromatin organization. P300 has been demonstrated to bind to phosphorylated CREB to mediate cAMP-gene regulation. P300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha). |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |