Rabbit anti-BIRC6/Apollon Antibody, Affinity Purified
SIGMA/PLA0098 - Powered by Bethyl Laboratories, Inc.
Synonym: BIR repeat-containing ubiquitin-
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-BIRC6/Apollon Antibody, Affinity Purified: Cat. No. PLA0098: Detection of Human BIRC6/Apollon by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-BIRC6/Apollon (Cat. No. PLA0098) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB staining.
Western Blotting Rabbit Anti-BIRC6/Apollon Antibody, Affinity Purified: Cat. No. PLA0098: Detection of Human BIRC6 by Western Blot and Immunoprecipitation. Samples: A) Whole cell lysate (WCL) and S100 extract (S100) from HeLa cells. B) Whole cell lysate (10 mg) from HeLa cells. Antibody: Affinity purified Rabbit Anti-BIRC6 Antibody used at 0.1 μg/mL for WB (A) and at 2 μg/mg lysate for IP (B). Detection: A) Chemiluminescence with an exposure time of 10 minutes. B) Immunoprecipitate was resolved by SDS-PAGE and stained with Coomassie Blue. The band that is BIRC6 was confirmed by mass spectrometry.
| accession no. | Q9NR09 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:2,000- 1:10,000 |
| immunoprecipitation (IP): 2-10 μg/mg | |
| western blot: 1:2,000-1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0098 maps to a region between residue 4775 and the C-terminus (residue 4829) of human Baculoviral IAP repeat-containing protein 6 using the numbering given in Swiss-Prot entry Q9NR09 (GeneID 57448). |
| Other Notes: | BIRC6/Apollon contains a baculoviral-IAP repeat (BIR) and ubiquitin-conjugating enzyme (UBC) domain and functions in the inhibition of apoptosis. BIRC6/Apollon inhibits apoptosis by ubiquitinating and promoting the proteasomal degradation of SMAC (second mitochondria-derived activator of caspase) and caspase-9. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |