Rabbit anti-XRN1 Antibody, Affinity Purified
SIGMA/PLA0105 - Powered by Bethyl Laboratories, Inc.
Synonym: SEP1; strand-exchange protein 1 homolog
Product Type: Product-on-demand
Western Blotting Rabbit Anti-XRN1 Antibody, Affinity Purified: Cat. No. PLA0105: Detection of Human XRN1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB) and 293T (Lane T; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-XRN1 antibody Cat. No. PLA0105 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
Western Blotting Rabbit Anti-XRN1 Antibody, Affinity Purified: Cat. No. PLA0105: Detection of Mouse XRN1 by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified Rabbit Anti-XRN1 Antibody PLA0105 used for WB at 0.2 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation Rabbit Anti-XRN1 Antibody, Affinity Purified: Cat. No. PLA0105: Detection of Human XRN1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-XRN1 antibody Cat. No. PLA0105 used for WB at 1 μg/mL and for IP at 3 μg/mg lysate. XRN1 was also immunoprecipitated by XRN1 Antibody B. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | Q6MZS8 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg/mg |
| western blot: 1:5,000- 1:15,000 | |
| UniProt accession no. | Q8IZH2  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0105 maps to a region between residue 1650 and the C-terminus (residue 1706) of human 5′-3′ Exoribonuclease 1 using the numbering given in TrEMBL entry Q6MZS8 (GeneID 54464). |
| Other Notes: | 5’-3’ exoribonuclease 1 (XRN1) is a ribonuclease localized in the cytoplasm that functions in mRNA metabolism and is found in messenger ribonucleoprotein (mRNP) complexes. The mouse and yeast homologs of XRN1 are Dhm2 and SEP1 respectively. In a glial cell line, XRN1 is induced by glial cell line–derived growth factor (GDNF) and associates with microtubules. XRN1 has also been identified as a candidate tumor suppressor gene in osteogenic sarcoma. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |