Rabbit anti-DDB1 Antibody, Affinity Purified
SIGMA/PLA0108 - Powered by Bethyl Laboratories, Inc.
Synonym: 127kDa; DDB p127 subunit; DDBA; DNA damage-binding protein a; Damage-specific DNA-binding protein 1; HBV X-associated protein 1; UV-DDB 1; UV-DDB1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; XAP-1; XAP1; XPCE; XPE; XPE-BF; XPE-binding factor; damage-specific DNA binding protein 1; damage-specific DNA binding protein 1 (127kD); xeroderma pigmentosum group E-complementing protein
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-DDB1 Antibody, Affinity Purified: Cat. No. PLA0108: Detection of Human and Mouse DDB1 by Immunohistochemistry. Sample: FFPE section of Human pancreatic islet cell tumor (left) and Mouse squamous cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-DDB1 (Cat. No. PLA0108) used at a dilution of 1:1,000 (0.2 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-DDB1 Antibody, Affinity Purified: Cat. No. PLA0108: Detection of Human and Mouse DDB1 by Western Blot and Human DDB1 by Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15, and 50 μg for WB; 1 mg for IP), Ramos (Lane R; 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibody: Affinity purified Rabbit Anti-DDB1 Antibody (Cat. No. PLA0108) used at 0.1 μg/mL for WB (A and B) and at 3 μg/mg lysate for IP. DDB1 was also immunoprecipitated using an affinity purified Antibody to an upstream epitope on DDB1. Immunoprecipitation reactions were controlled using normal Rabbit IgG (Ctrl. IgG). Detection: Chemiluminescence with an exposure time of 30 seconds (A and B).
| accession no. | Q13289 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.2 mg/mL |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500- 1:2,000 |
| immunoprecipitation (IP): 2-5 μg/mg | |
| western blot: 1:1,000- 1:10,000 | |
| UniProt accession no. | Q16531  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0108 maps to a region between residue 1100 and the C-terminus (residue 1140) of human damage-specific DNA binding protein 1 using the numbering given in TrEMBL entry Q13289 (GeneID 1642). |
| Other Notes: | DDB1 (DNA Damage Binding Protein 1) is a subunit of the DNA damage-binding protein complex (DDB) involved in DNA repair. As part of a complex with Cul4-based ubiquitin ligase, Cul4-Ddb1 can directly modify chromatin through the ubiquitination of histones as well as ubiquitinate other chromatin bound factors. A deficiency in Cul4-Ddb1 ubiquitin ligase has been associated with xeroderma pigmentosum, a heritable disease characterized by defects in nucleotide excision repair and skin cancer development. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |