Rabbit anti-CRM1 Antibody, Affinity Purified
SIGMA/PLA0109 - Powered by Bethyl Laboratories, Inc.
Synonym: CRM1; chromosome region maintenance 1 homolog; chromosome region maintenance 1 homolog (yeast); chromosome region maintenance 1 protein homolog; emb; exp1; exportin 1 (CRM1 homolog; yeast); exportin 1 (CRM1; yeast; homolog); exportin-1 (required for chromosome region maintenance)
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-CRM1 Antibody, Affinity Purified: Cat. No. PLA0109: Detection of Human CRM1 by Immunohistochemistry. Sample: FFPE section of Human metastatic lymph node. Antibody: Affinity purified Rabbit Anti-CRM1 (Cat. No. PLA0109) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-CRM1 Antibody, Affinity Purified: Cat. No. PLA0109: Detection of CRM1 (Exportin 1) by Western Blot and Immunoprecipitation. Samples: A) Whole cell lysate from HeLa (5, 15 and 50 μg), Ramos (50 μg) and Mouse NIH3T3 (50 μg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-CRM1 Antibody (Cat. No. PLA0109) used at 0.1 μg/mL for WB (A and B) and at 3 μg/mg lysate for IP. CRM1 was also immunoprecipitated by Rabbit Anti-CRM1 Antibodies which recognize upstream epitopes on CRM1. Detection: Chemiluminescence with an exposure time of 3 seconds (A and B).
| accession no. | NP_003391.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:1,000-1:5,000 |
| immunoprecipitation (IP): 2-10 μg/mg | |
| western blot: 1:2,000-1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0109 maps to a region between residue 1025 and the C-terminus (residue 1071) of human Chromosome Region Maintenance 1 (Exportin 1) using the numbering given in entry NP_003391.1 (GeneID 7514). |
| Other Notes: | Chromosome region maintenance 1 protein homolog (CRM1) is the Ran-GTP-binding nuclear export receptor for proteins bearing a leucine rich nuclear export signal and RNAs. CRM1 also possesses a mitotic function as it localizes to kinetochores, and with Ran-GTP, recruits Ran-GTPase-activating protein (Ran-GAP1) and Ran-binding protein (Ran-BP2) for proper mitotic spindle assembly and chromosome segregation. Additionally, CRM1 is suggested to function as a loading dock of various cell cycle and checkpoint factors such as cyclins, Cdks, p53 and BRCA1, to regulate proper chromosome duplication and genomic stability. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |