Rabbit anti-Lasu1/Ureb1 Antibody, Affinity Purified
SIGMA/PLA0111 - Powered by Bethyl Laboratories, Inc.
Synonym: ARF-BP1; ARF-binding protein 1; BJ-HCC-24 tumor antigen; HECT; HECT; HECT domain protein LASU1; HECTH9; HSPC272; Ib772; KIAA0312; LASU1; MULE; Mcl-1 ubiquitin ligase E3; UBA and WWE domain containing 1; UBA and WWE domain-containing protein 1; URE-B1; URE-binding protein 1; UREB1; homologous to E6AP carboxyl terminus homologous protein 9; large structure of UREB1; upstream regulatory element-binding protein 1
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-Lasu1/Ureb1 Antibody, Affinity Purified: Cat. No. PLA0111: Detection of Human Lasu1/Ureb1 by Immunohistochemistry. Sample: FFPE section of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-Lasu1/Ureb1 (Cat. No. PLA0111) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-Lasu1/Ureb1 Antibody, Affinity Purified: Cat. No. PLA0111: Detection of Human Ureb1/Lasu1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), and HeLa (50 μg) cells. Antibodies: Affinity purified Rabbit Anti-Ureb1/Lasu1 Antibody PLA0111 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blotting Rabbit Anti-Lasu1/Ureb1 Antibody, Affinity Purified: Cat. No. PLA0111: Detection of Mouse Ureb1/Lasu1 by Western Blot. Samples: Whole cell lysate (50 μg) from TCMK-1, Renca, 4T1, and CT26.WT cells. Antibodies: Affinity purified Rabbit Anti-Ureb1/Lasu1 Antibody PLA0111 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation Rabbit Anti-Lasu1/Ureb1 Antibody, Affinity Purified: Cat. No. PLA0111: Detection of Human Ureb1/Lasu1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified Rabbit Anti-Ureb1/Lasu1 antibody Cat. No. PLA0111 used for IP at 6 μg/mg lysate. For blotting immunoprecipitated Ureb1/Lasu1, PLA0111 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
| accession no. | NP_113584.3 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:1000-1:5,000 |
| immunoprecipitation (IP): 2-10 μg/mg | |
| western blot: 1:2,000-1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0111 maps to a region between residues 1025 and 1075 of human Upstream Regulatory Element Binding Protein 1 using the numbering given in entry NP_113584.2 (GeneID 10075). |
| Other Notes: | The conjugation of ubiquitin to proteins is an important means to regulate protein activity for many cellular processes by tagging them for degradation. Lasu1/Ureb1 is an E3 ubiquitin protein ligase that mediates the ubiquitination of core histones, Mcl-1, Cdc6, and p53. Lasu1/Ureb1 expression is regulated during spermatogenesis and appears to play a role in chromatin modification in germ cells as well as other tissues. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |