Rabbit anti-CtIP Antibody, Affinity Purified
SIGMA/PLA0112 - Powered by Bethyl Laboratories, Inc.
Synonym: COM1; CTBP-interacting protein; CtIP; JWDS; RBBP-8; RIM; Retinoblastoma-
Product Type: Product-on-demand
Western Blotting Rabbit Anti-CtIP Antibody, Affinity Purified: Cat. No. PLA0112: Detection of Human CtIP by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 μg), 293T (Lane T; 50 μg) and Jurkat (Lane J; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-CtIP antibody Cat. No. PLA0112 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with exposure time of 3 minutes.
Western Blotting Rabbit Anti-CtIP Antibody, Affinity Purified: Cat. No. PLA0112: Detection of Mouse CtIP by Western Blot. Samples: Whole cell lysate (50 μg) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified Rabbit Anti-CtIP Antibody PLA0112 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation Rabbit Anti-CtIP Antibody, Affinity Purified: Detection of Human CtIP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-CtIP antibody Cat. No. PLA0112 used for WB at 1 μg/mL and used for IP at 3 μg/mg lysate. CtIP was also immunoprecipitated by CtIP Antibody B. Detection: Chemiluminescence with exposure time of 3 minutes.
| accession no. | NP_002885.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-10 μg/mg |
| western blot: 1:2,000-1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0112 maps to a region between residue 850 and the C-terminus (residue 897) of human CtBP Interacting Protein (Retinoblastoma Binding Protein 8) using the numbering given in entry NP_002885.1 (GeneID 5932). |
| Other Notes: | CtIP (CTBP interacting protein) was first identified as a protein that interacted with CTBP (C-terminus-binding protein of adenovirus E1A). CtIP has also been identified as a protein that interacts with the tumor suppressors pRb and BRCA1 and has been implicated as a tumor suppressor itself. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |