Rabbit anti-Estrogen Receptor Alpha Antibody, Affinity Purified
SIGMA/PLA0113 - Powered by Bethyl Laboratories, Inc.
Synonym: CTERP-1; ER; ER-alpha; ERalphaDup5; ERalphai45a; ERalphai45bL; ERalphai45bS; ERalphai45c; ERalphai56; ERalphai67; ESR; ESRA; ESTRR; Era; NR3A1; estradiol receptor; estrogen nuclear receptor alpha; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant; estrogen receptor alpha splice variant; estrogen receptor alpha splice variant; estrogen receptor alpha splice variant; estrogen receptor alpha splice variant; estrogen receptor alpha splice variant; estrogen receptor alpha splice variant; estrogen receptor alpha splice variant; nuclear receptor subfamily 3 group A member 1
Product Type: Product-on-demand
Western Blotting Rabbit Anti-Estrogen Receptor Alpha Antibody, Affinity Purified: Cat. No. PLA0113: Detection of Human Estrogen Receptor Alpha by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 μg for WB; 1 mg for IP, 1/4 of IP loaded) from MCF7 cells. Antibodies: Affinity purified Rabbit Anti-ER alpha Antibody (Cat. No. PLA0113) used at 0.1 μg/mL for WB (A and B) and at 3 μg/mg lysate for IP (B). ER alpha was also immunoprecipitated by Rabbit Anti-ER alpha Antibodies recognizing different epitopes using each at 3 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
| accession no. | P03372 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-10 μg/mg |
| western blot: 1:2,000-1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0113 maps to a region between residue 550 and the C-terminus (residue 595) of human Estrogen Receptor Alpha using the numbering given in SwissProt entry P03372 (GeneID 2099). |
| Other Notes: | The estrogen receptor (ER) is the steroid-hormone nuclear receptor that binds estrogen and functions to activate genes involved in a variety of biological processes. Two forms of the ER, ER alpha and ER beta, are encoded by independent genes, and each plays different roles in gene regulation. Additional variants of ER beta have also been reported; these include ERb2, ERb3, ERb4, and ERb5. All forms of the ER have the ability to heterodimerize with each other. Differences in localization and concentrations as well as heterodimerization among the variants may serve to achieve distinct cell and tissue responses. ER alpha generally functions as an activator of transcription while ER beta functions as a repressor. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |