Rabbit anti-NONO Antibody, Affinity Purified
SIGMA/PLA0119 - Powered by Bethyl Laboratories, Inc.
Synonym: 52 kDa subunit; 54 kDa nuclear RNA- and DNA-binding protein; 54-kD; 55 kDa nuclear protein; DNA-binding p52/p100 complex; MRXS34; NMT55; NRB54; NonO protein; Nuclear RNA-binding protein; P54; P54NRB; PPP1R114; non-Pou domain-containing octamer (ATGCAAAT) binding protein; p54(nrb); protein phosphatase 1; regulatory subunit 114
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-NONO Antibody, Affinity Purified: Cat. No. PLA0119: Detection of Human and Mouse NONO by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma (left) and Mouse squamous cell carcinoma (right). Antibody: Affinity purified Rabbit Anti- NONO (Cat. No. PLA0119) used at a dilution of 1:1,000 (0.2 μg/mL) and 1:200 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-NONO Antibody, Affinity Purified: Cat. No. PLA0119: Detection of Human NONO by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 1/4 of IP reaction loaded) and 293T (T; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-NONO Antibody (Cat. No. PLA0119) used for WB at 0.04 μg/mL (A) and 1 μg/mL (B) and used for IP at 3 μg/mg lysate. NONO was also immunoprecipitated using an Anti-NONO Antibody that recognizes a different epitope at 3 μg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Western Blotting Rabbit Anti-NONO Antibody, Affinity Purified: Cat. No. PLA0119: Detection of Mouse and Rat NONO by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified Rabbit Anti-NONO Antibody PLA0119 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
| accession no. | NP_031389.3 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200-1:1,000 |
| immunoprecipitation (IP): 1- 4 μg/mg | |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | Q15233  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0119 maps to a region between residues 350 and 400 of human Non-POU Domain Containing, Octamer-binding using the numbering given in entry NP_031389.3 (GeneID 4841). |
| Other Notes: | Non-POU-domain-containing octamer binding protein (NONO) is a member of the DBHS (drosophila behavior, human splicing) domain-containing family and is an RNA- and DNA- binding protein. NONO and other DBHS domain-containing proteins are multifunctional and are reported to be involved in transcriptional regulation, mRNA processing, and DNA non-homologous end joining (NHEJ). NONO functions as a coregulator of the androgen receptor (AR) and also regulates cAMP transcriptional activity by interacting with gene promoter elements. NONO is also involved in pre-mRNA splicing through an interaction with U5 snRNA and can stimulate DNA nonhomologous end joining (NHEJ) through the interaction with ku70/G22p and ku80/XRCC5 dimers. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |