Rabbit anti-ErbB2 Antibody, Affinity Purified
SIGMA/PLA0123 - Powered by Bethyl Laboratories, Inc.
Synonym: CD antigen CD340; CD340; HER-2; HER-2/neu; HER2; MLN 19; NEU; NGL; TKR1; c-erb B2/neu protein; herstatin; human epidermal growth factor receptor 2; metastatic lymph node gene 19 protein; neuro/glioblastoma derived oncogene homolog; neuroblastoma/
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-ErbB2 Antibody, Affinity Purified: Cat. No. PLA0123: Detection of Human ErbB2 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-ErbB2 (Cat. No. PLA0123) used at a dilution of 1:200 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-ErbB2 Antibody, Affinity Purified: Cat. No. PLA0123: Detection of Human and Mouse ErbB2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg for WB) and Mouse NIH3T3 (Lane M; 50 μg for WB) cells. Antibodies: Affinity purified Rabbit Anti-ErbB2 Antibody (Cat. No. PLA0123) used for WB at 1 μg/mL (A) and 0.04 μg/mL (B) and used for IP at 3 μg/mg lysate. ErbB2 was also immunoprecipitated using Rabbit Anti-ErbB2 Antibodies recognizing different epitopes at 3 μg/mg lysate. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
| accession no. | NP_004439.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:100- 1:500 |
| immunoprecipitation (IP): 1-4 μg/mg | |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | P04626  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0123 maps to a region between residue 1200 and the C-terminus (residue 1255) of human v-erb-b2 erythroblastic leukemia viral oncogene homolog 2 using the numbering given in entry NP_004439.2 (GeneID 2064). |
| Other Notes: | ErbB2 is a member of the receptor tyrosine kinase family of proteins. The four members of this family are ErbB1 (Epidermal growth factor receptor; EGFR/HER1), ErbB2 (HER2/Neu), ErbB3 (HER3), and ErbB4 (HER4). The ErbB receptor members are single-pass transmembrane proteins with an extracellular ligand-binding domain and a cytoplasmic tyrosine-kinase domain. The epidermal growth factor (EGF) and its related proteins serve as the ligands for the ErbB receptors. Upon binding, the receptors heterodimerize, autophosphorylate and make contact with adapter molecules to transmit cellular signals involved in cell proliferation, survival, and differentiation. The ErbB2 receptor is the only member that does not interact with a ligand, but instead functions as a heterodimeric partner to coordinate signals from other ligand bound ErbB receptors. Amplification and/or overexpression of ErbB2 have been reported in many cancers. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |