Rabbit anti-GAPDH Antibody, Affinity Purified
SIGMA/PLA0125 - Powered by Bethyl Laboratories, Inc.
Synonym: G3PD; GAPD; GAPDH; HEL-S-162eP; aging-associated gene 9 protein; epididymis secretory sperm binding protein Li 162eP; peptidyl-cysteine S-nitrosylase GAPDH
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-GAPDH Antibody, Affinity Purified: Cat. No. PLA0125: Detection of Human and Mouse GAPDH by Immunohistochemistry. Sample: FFPE section of Human lung carcinoma (left) and Mouse squamous cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-GAPDH (Cat. No. PLA0125) used at a dilution of 1:200 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-GAPDH Antibody, Affinity Purified: Cat. No. PLA0125: Detection of Human and Mouse GAPDH by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg) and Mouse NIH3T3 (Lot M; 50 μg). Antibody: Affinity purified Rabbit Anti-GAPDH Antibody (Cat. No. PLA0125) used at 0.04 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
| accession no. | NP_002037.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:100-1:500 |
| western blot: 1:10,000-1:25,000 | |
| UniProt accession no. | P04406  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0125 maps to a region between residues 250 and 300 of human Glyceraldehyde-3-Phosphat |
| Other Notes: | In the process of carbohydrate metabolism, GAPDH (glyceraldehydes-3-phosph |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |