Rabbit anti-Phospho 53BP1 (S25) Antibody, Affinity Purified
SIGMA/PLA0126 - Powered by Bethyl Laboratories, Inc.
Synonym: 1; 1; 53BP1; TDRD30; TP53; p202; p53-binding protein 1; p53BP1; tumor protein 53-binding protein; tumor protein p53-binding protein
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-Phospho 53BP1 (S25) Antibody, Affinity Purified: Cat. No. PLA0126: Detection of Human and Mouse Phospho 53BP1 (S25) by Immunohistochemistry. Sample: FFPE sections of Human stomach carcinoma (left) and Mouse teratoma (right). Antibody: Affinity purified Rabbit Anti-Phospho 53bp1 (S25) (Cat. No. PLA0126 ) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-Phospho 53BP1 (S25) Antibody, Affinity Purified: Cat. No. PLA0126: Detection of Phospho 53BP1 (S25) by Western Blot. Samples: Whole cell lysate (30 μg or 15 μg for WB; 1 mg for IP, 20% of IP loaded) from 293T cells that were mock treated or treated with NCS (200 ng/mL; 1 hour). Antibodies: Affinity purified Rabbit Anti-Phospho 53BP1 Antibody PLA0126 at 0.04 μg/mL (A) and 0.1 μg/mL (B) for WB. 53BP1 was also immunoprecipitated using an affinity purified Rabbit Anti-53BP1 Antibody that recognizes a different epitope. Detection: Chemiluminescence with exposure times of 3 seconds (A and B).
Flow Cytometry Rabbit Anti-Phospho 53BP1 (S25) Antibody, Affinity Purified: Cat. No. PLA0126: Flow cytometric analysis of phospho 53bp1 (S25). Jurkat cells were treated with neocarzinostatin (NCS) for 1.5 hours. 1 × 106 cells were fixed, permeabilized, and stained with 1.5 μg/mL Anti-phospho 53bp1 (S25) PLA0126 in a 150 mcl reaction. Isotype control (black), untreated (red), NCS treated (blue).
| accession no. | NP_005648.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | phosphorylation (pSer25 ) |
| technique(s) | immunohistochemistry: 1:1,000- 1:5,000 |
| immunoprecipitation (IP): 1- 4 μg/mg | |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Immunogen for PLA0126 was a phosphorylated synthetic peptide, which represented a portion of human p53 Binding Protein 1 (GeneID 7158) around serine 25 according to the numbering given in entry NP_005648.1. |
| Other Notes: | 53BP1 (p53 Binding Protein 1) is an early participant in the cellular response to DNA double-strand breaks. 53BP1 functions in a redundant role in phosphorylation of the downstream checkpoint effector proteins Brca1 and Chk2 but was required for the formation of Brca1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |