Rabbit anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified
SIGMA/PLA0141 - Powered by Bethyl Laboratories, Inc.
Synonym: BM28; CCNL1; CDCL1; D3S3194; DFNA70; KIAA0030; MCM2 minichromosome maintenance deficient 2; cdc19; cell devision cycle-like 1; cyclin-like 1; minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin); minichromosome maintenance deficient 2 (mitotin); minichromosome maintenance protein 2 homolog; mitotin; mitotin (S. cerevisiae); nuclear protein BM28
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified: Cat. No. PLA0141: Detection of Human Phospho MCM2 (S40/S41) by Immunohistochemistry. Samples: FFPE serial sections of Human colon carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified Rabbit Anti-Phospho MCM2 (S40/S41) (Cat. No. PLA0141) used at a dilution of 1:200 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified: Cat. No. PLA0141: Detection of Human Phospho MCM2 (S40/S41) by Western Blot. Samples: Whole cell lysate (50 μg) from asynchronous 293T cells that was mock treated (-) or treated (+) with phosphatases (PPase). Antibody: Affinity purified Rabbit Anti-phospho MCM2 (S40/S41) Antibody (Cat. No. PLA0141) used at 0.1 μg/mL. To examine total MCM2, the blot was stripped and then blotted with Rabbit Anti-MCM2 Antibody (Cat. No. PLA0060) at 0.1 μg/mL. Detection: Chemiluminescence with exposure times of 30 seconds (upper and lower panels).
Flow Cytometry Rabbit Anti-Phospho MCM2 (S40/S41) Antibody, Affinity Purified: Cat. No. PLA0141: Flow cytometric analysis of phospho-MCM2 (pS40/41). Jurkat cells were fixed in 1.5% PFA, and permeabilized in 90% MeOH. 1 × 106 cells were stained with 0.1 μg/mL Anti-phospho-MCM2 (pS40/41) [PLA0141] in a 150 mcl volume. DNA content was simultaneously analyzed via PI stain.
| accession no. | NP_004517.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | phosphorylation (pSer40/pSer41) |
| technique(s) | flow cytometry: 0.1 μg |
| immunohistochemistry: 1:100- 1:500 | |
| western blot: 1:1,000- 1:5,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Immunogen was a synthetic di-phosphorylated peptide, which represented a portion of human minichromosomal maintenance deficient 2 surrounding Serines 40 and 41 according to the numbering given in entry NP_004517.2 (GeneID 4171). |
| Other Notes: | The MCM (Mini-Chromosome Maintenance) complex is a key component of the pre-replication complex involved in replication licensing which restricts DNA replication to only once per cell cycle. The MCM complex is a heterohexamer of MCM2, MCM3, MCM4, MCM5, MCM6, and MCM7. MCM2 interacts with the core MCM complex (MCM4, 6, and 7) to regulate its helicase activity. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |