Rabbit anti-BRG1/SMARCA4 Antibody, Affinity Purified
SIGMA/PLA0146 - Powered by Bethyl Laboratories, Inc.
Synonym: ATP-dependent helicase SMARCA4; BAF190; BAF190A; BRG1; BRG1-associated factor 190A; BRM/SWI2-related gene 1; CSS4; FLJ39786; MRD16; RTPS2; SNF2; SNF2-BETA; SNF2-like 4; SNF2L4; SNF2LB; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; SWI2; brahma protein-like 1; global transcription activator homologous sequence; hSNF2b; homeotic gene regulator; mitotic growth and transcription activator; nuclear protein GRB1; protein BRG-1; protein brahma homolog 1; sucrose nonfermenting-like 4
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-BRG1/SMARCA4 Antibody, Affinity Purified: Cat. No. PLA0146: Detection of Human and Mouse BRG1/SMARCA4 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma (left) and Mouse colon carcinoma CT26. Antibody: Affinity purified Rabbit Anti-BRG1/SMARCA4 (Cat. No. PLA0146) used at a dilution of 1:500 (0.4 μg/mL) and 1:250 (0.8 μg/mL). Detection: DAB.
Immunohistochemistry Rabbit Anti-BRG1/SMARCA4 Antibody, Affinity Purified: Cat. No. PLA0146: Detection of Human BRG1/SMARCA4 by Immunohistochemistry. Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified Rabbit Anti-BRG1/SMARCA4 Cat. No. PLA0146 used at a dilution of 1:1,000 (0.2μg/mL). Detection: DAB
Immunohistochemistry Rabbit Anti-BRG1/SMARCA4 Antibody, Affinity Purified: Cat. No. PLA0146: Detection of Mouse BRG1/SMARCA4 by Immunohistochemistry. Sample: FFPE section of Mouse plasmacytoma. Antibody: Affinity purified Rabbit Anti-BRG1/SMARCA4 Cat. No. PLA0146 used at a dilution of 1:1,000 (0.2μg/mL). Detection: DAB
Western Blotting Rabbit Anti-BRG1/SMARCA4 Antibody, Affinity Purified: Cat. No. PLA0146: Detection of Human BRG1/SMARCA4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BRG1/SMARCA4 antibody Cat. No. PLA0146 used for IP at 6 μg per reaction. BRG1/SMARCA4 was also immunoprecipitated by BRG1/SMARCA4 Ab B. For blotting immunoprecipitated BRG1/SMARCA4, PLA0146 was used at 0.04 μg/mL. Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation Rabbit Anti-BRG1/SMARCA4 Antibody, Affinity Purified: Cat. No. PLA0146: Detection of Human BRG1/SMARCA4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BRG1/SMARCA4 antibody Cat. No. PLA0146 used for IP at 6 μg per reaction. BRG1/SMARCA4 was also immunoprecipitated by BRG1/SMARCA4 Ab B. For blotting immunoprecipitated BRG1/SMARCA4, PLA0146 was used at 0.04 μg/mL. Chemiluminescence with an exposure time of 3 minutes.
| accession no. | NP_003063.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:250- 1:2,000 |
| immunoprecipitation (IP): 2-10 μg/mg | |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0146 maps to a region between residue 75 and 125 of human BRG1/SMARCA4 using the numbering given in entry NP_003063.2 (GeneID 6597). |
| Other Notes: | The SWI/SNF-related, matrix-associated, actin-dependent regulators of chromatin (SMARC), also called BRG1-associated factors (BAFs), have been identified as components of the human SWI/SNF-like chromatin-remodeling protein complexes. BRG1/SMARCA4 is an ATPase subunit of the SWI/SNF-A chromatin remodeling complex (BAF complex). In BAF complexes, BRG1/SMARCA4 has been shown to interact with BRCA1. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA with 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |