Rabbit anti-Histone H3 Antibody, Affinity Purified
SIGMA/PLA0148 - Powered by Bethyl Laboratories, Inc.
Synonym: H3 histone family; H3/A; H3FA; H3a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; histone 1; histone H3/a; member A
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-Histone H3 Antibody, Affinity Purified: Cat. No. PLA0148: Detection of Mouse Histone H3 by Immunohistochemistry. Sample: FFPE section of Mouse teratoma. Antibody: Affinity purified Rabbit Anti-Histone H3 (Cat. No. PLA0148) used at a dilution of 1:1,000 (0.2 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-Histone H3 Antibody, Affinity Purified: Cat. No. PLA0148: Detection of Human Histone H3 by Western Blot and Immunoprecipitation. Samples: Histones (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded) isolated from 293T cells. Antibodies: Affinity purified Rabbit Anti-Histone H3 Antibody (Cat. No. PLA0148) used for WB at 0.04 μg/mL (A) and 1 μg/mL (B) and used for IP at 3 μg/mg histones (B). Histone H3 was also immunoprecipitated by a Rabbit Anti-Histone H3 Antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 1 second (A and B).
| accession no. | NP_003520.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200-1:1,000 |
| immunoprecipitation (IP): 2-10 μg/mg | |
| western blot: 1:2,000- 1:10,000 |
| Biochem/physiol Actions: | Histone proteins are the basic building blocks of chromatin. Histone H3 controls protein-protein interactions to induce binding of trans-acting factors that have a role in chromatin condensation. Histone H3 K27M mutations are observed in adult cerebellar high-grade gliomas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Mammals contain variants of histone H3, that are, the replicative histones (H3.1 and H3.2) and the replacement histones (H3.3). Histone H3 is located on human chromosome 6p22. |
| Immunogen: | The epitope recognized by PLA0148 maps to a region between residue 100 and the C-terminus (residue 135) of human Histone H3 using the numbering given in entry NP_003520.1 (GeneID 8350). |
| Other Notes: | Histone H3 is one of four histone proteins that assemble into an octamer to form the nucleosome core and serve to spool and compact DNA into chromatin. Histones are subject to a complex set of post-translational modifications (acetylation, methylation, phosphorylation, ubiquitination, sumoylation, citrullination, and ADP ribosylation) that are important to the regulation of gene transcription, DNA replication, and DNA repair. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA with 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |