Rabbit anti-CBX5 Antibody, Affinity Purified
SIGMA/PLA0150 - Powered by Bethyl Laboratories, Inc.
Synonym: HEL25; HP1; HP1 alpha; HP1 alpha homolog; HP1 alpha homolog (Drosophila); HP1-ALPHA; HP1A; HP1Hs alpha; HP1Hs-alpha; antigen p25; chromobox homolog 5; chromobox homolog 5 (Drosophila HP1 alpha); chromobox homolog 5 (HP1 alpha homolog; Drosophila); epididymis luminal protein 25; heterochromatin protein 1 homolog alpha; heterochromatin protein 1-alpha
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-CBX5 Antibody, Affinity Purified: Cat. No. PLA0150: Detection of Human CBX5 by Immunohistochemistry. Sample: FFPE section of Human prostate carcinoma. Antibody: Affinity purified Rabbit Anti-CBX5 (Cat. No. PLA0150) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB staining.
Western Blotting Rabbit Anti-CBX5 Antibody, Affinity Purified: Cat. No. PLA0150: Detection of Human CBX5 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-CBX5 Antibody (Cat. No. PLA0150) used for WB at 0.1 μg/mL (A) and 1 μg/mL (B) and used for IP at 3 μg/mg lysate (B). Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
| accession no. | NP_036249.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:2,000- 1:10,000 |
| immunoprecipitation (IP): 2- 5 μg/mg | |
| western blot: 1:1,000-1:10,000 | |
| UniProt accession no. | P45973  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0150 maps to a region between residue 150 and the C-terminus (residue 191) of human Chromobox Homolog 5 using the numbering given in entry NP_036249.1 (GeneID 23468). |
| Other Notes: | CBX5 (chromobox protein homolog 5), also known as HP1 (heterochromatin protein 1 homolog alpha), is one of 3 isoforms of HP1 that binds heterochromatin and functions as a structural adaptor to stabilize and organize macromolecular complexes with nucleosomes for higher chromatin structure. All of the HP1 isoforms possess a chromodomain and chromoshadow domain and associate with methylated histones that mediate transcriptional repression. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |