Rabbit anti-PPP1CA Antibody, Affinity Purified
SIGMA/PLA0154 - Powered by Bethyl Laboratories, Inc.
Synonym: PP-1A; PP1A; PP1alpha; PPP1A; alpha isoform; alpha isozyme; catalytic subunit; catalytic subunit; protein phosphatase 1; protein phosphatase 1; serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit; testicular tissue protein Li 155
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-PPP1CA Antibody, Affinity Purified: Cat. No. PLA0154: Detection of Human PPP1CA by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-PPP1CA (Cat. No. PLA0154) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-PPP1CA Antibody, Affinity Purified: Cat. No. PLA0154: Detection of Human and Mouse PPP1CA by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, Jurkat, K562, Mouse TCMK-1, and Mouse NIH3T3 cells. Antibodies: Affinity purified Rabbit Anti-PPP1CA Antibody PLA0154 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
| accession no. | NP_002699.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500- 1:2,000 |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | P62136  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0154 maps to a region between residue 280 and the C-terminus (residue 330) of human Protein Phosphatase 1, Catalytic Subunit, Alpha Isoform using the numbering given in entry NP_002699.1 (GeneID 5499). |
| Other Notes: | Protein phosphatase 1 (PP1) is a major eukaryotic serine/threonine phosphatase that is involved in a multitude of cellular functions. PP1 is a holoenzyme that consists of the PP1 catalytic subunit (PP1c) that associates with and is regulated by over 50 regulatory subunits. There are several gene and alternative splice products of the catalytic subunit PP1c. PPP1CA encodes the PP1 alpha catalytic subunit. In addition to PP1 alpha, there is PP1 beta (PPP1CB), and two splice variants of PP1 gamma (PPP1CC). The mutually exclusive interaction between the various catalytic and regulatory subunits allows the PP1 enzyme to be involved in a variety of functions which include cell division, glycogen metabolism, muscle contractility, and protein synthesis. serine/threonine-protein phosphatase PP1-alpha catalytic subunit, PPP1A, PP-1A, MGC1674, MGC15877. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |