Rabbit anti-ATRX Antibody, Affinity Purified
SIGMA/PLA0163 - Powered by Bethyl Laboratories, Inc.
Synonym: ATP-dependent helicase ATRX; ATR2; DNA dependent ATPase and helicase; JMS; Juberg-Marsidi syndrome; MRX52; MRXHF1; RAD54; RAD54 homolog (S. cerevisiae); RAD54L; SFM1; SHS; X-linked; X-linked 52; X-linked helicase II; X-linked nuclear protein; XH2; XNP; ZNF-HX; Zinc finger helicase; alpha thalassemia/mental retardation syndrome X-linked (RAD54 (S. cerevisiae) homolog); alpha thalassemia/mental retardation syndrome X-linked (RAD54 homolog. S. cerevisiae); helicase 2; mental retardation
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-ATRX Antibody, Affinity Purified: Cat. No. PLA0163: Detection of Human ATRX by Immunohistochemistry. Sample: FFPE section of Human prostate carcinoma. Antibody: Affinity purified Rabbit Anti-ATRX (Cat. No. PLA0163) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.
Western Blotting Rabbit Anti-ATRX Antibody, Affinity Purified: Cat. No. PLA0163: Detection of Human ATRX by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit Anti-ATRX antibody Cat. No. PLA0163 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation Rabbit Anti-ATRX Antibody, Affinity Purified: Cat. No. PLA0163: Detection of Human ATRX by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit Anti-ATRX antibody Cat. No. PLA0163 used for IP at 6 μg per reaction. ATRX was also immunoprecipitated by Rabbit Anti-ATRX Antibody B. For blotting immunoprecipitated ATRX, PLA0163 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
| accession no. | NP_000480.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry: 1:500-1:2,000 |
| immunoprecipitation (IP): 2-10 μg/mg | |
| western blot: 1:1,000-1:5,000 | |
| UniProt accession no. | P46100  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0163 maps to a region between residue 2442 and the C-terminus (residue 2492) of human alpha thalassemia/mental retardation syndrome X-linked using the numbering given in entry NP_000480.2 (GeneID 546). |
| Other Notes: | ATRX (alpha-thalassemia mental retardation X-linked) is a chromatin remodeling protein and transacting regulator of globin gene expression that has been linked to several X-linked mental retardation syndromes and hematologic diseases such as myelodysplasia. ATRX possesses an ATPase/helicase domain and is related to the SWI/SNF family of proteins. Mutations in ATRX have been demonstrated to cause changes in DNA methylation patterns and gene expression important to development. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |