Rabbit anti-ATAD5/FRAG1 Antibody, Affinity Purified
SIGMA/PLA0164
Synonym: C17orf41; ELG1; FLJ12735; FRAG1; chromosome 17 open reading frame 41; chromosome fragility associated gene 1; chromosome fragility-associated gene 1 protein; enhanced level of genomic instability 1 homolog; enhanced level of genomic instability 1 homolog (S. cerevisiae)
Product Type: Product-on-demand
Western Blotting Rabbit Anti-ATAD5/FRAG1 Antibody, Affinity Purified: Cat. No. PLA0164: Detection of Human ATAD5/FRAG1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 μg) from HeLa and 293T (Lane T; 50 μg) cells. Antibody: Affinity purified Rabbit Anti-ATAD5/FRAG1 antibody Cat. No. PLA0164 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
Immunoprecipitation Detection of Human ATAD5/FRAG1 by Western Blot of Immunoprecipitate. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified Rabbit Anti-ATAD5/FRAG1 antibody Cat. No. PLA0164 used for WB at 1 μg/mL and for IP at 3 μg/mg lysate. Detection: Chemiluminescence with exposure time of 30 seconds.
| accession no. | NP_079133.3 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2- 5 μg/mg |
| proximity ligation assay: suitable | |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0164 maps to a region between residue 775 and 825 of human ATPase family, AAA domain containing 5 using the numbering given in entry NP_079133.3 (GeneID 79915). |
| Other Notes: | ATAD5/FRAG1 has been identified as a homolog of a subunit of RFC (replication factor C) and has been shown to be down-regulated in response to replicative stress. ATAD5/FRAG1 may play a role in the surveillance of DNA damage and the cell’s decision to survive or activate apoptosis. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |